Padilla's parents divorced when he was two years old, which he has said did not greatly affect him. According to his video "Draw My Life," he has two younger brothers.
By his own account, Padilla has said that he was very introverted as a child, due in part to anxiety issues that would affect him when he received attention from others. He has also spoken about having aspirations to get into game design when he was a child.
Padilla suffers from anxiety, in which he experiences panic attacks often. He has said that due to his mother having a similar issue, he believes this condition to be hereditary in his family.
Padilla began his YouTube career in 2005, posting to the Smosh channel with long-time YouTube co-creator and friend Ian Hecox. The duo originally posted lip-sync videos, a common video type at the time which featured the stars lip-sync singing a particular song whilst performing entertaining or comedic gestures. The earliest of their lip-sync videos, "Mortal Kombat Theme" and "Pokemon Theme" gained significant notoriety, both appearing on YouTube Front Page (a now-defunct feature similar in functionality to YouTube Spotlight).
The success of their lip-sync videos garnered a significant following for Padilla and Hecox, inspiring them to write and produce more detailed comedic videos, which primarily involved various skits. The skits they created as early as 2006 set the stage for their continued success on YouTube.
In addition to the Smosh channel and all associated channels, Padilla concurrently operated his own self-named channel from 2006 to 2013. In 2013, the AnthonyPadilla channel took an indefinite hiatus. Padilla did not comment as to whether or not the channel will continue.
Anthony's final video on his personal channel mentioned he will be doing daily videos on his snapchat in 2015. His snapchat name is anth0nypadilla.
He returned to uploading videos on the channel in 2016, uploading vlogs which at times consisted of his current girlfriend, Miel.
Since leaving Smosh in 2017, he has primarily uploaded sketches and vlogs which at times features guest stars, usually other YouTube stars, every Friday.
List Of Subscriber Milestones
- 1 million subscribers: August 8, 2013
- 2 million subscribers: June 20, 2017
Padilla has had multiple relationships during his YouTube stardom, but many of them have been predominantly private and rather unknown.
Padilla began dating fellow YouTuber Kalel Cullen in 2010. The couple became engaged in 2013, when they launched their WatchUsLiveAndStuff channel; a daily account of their life. In December 2014, Padilla and Cullen announced via YouTube that they had decided to split, citing irreconcilable differences. However, in his recently uploaded Draw My Life video, he says that Kalel manipulated and used him, although he did not specifically say her name at all in the video. He also goes into greater detail about his anxiety and panic attacks, life as a child with divorced parents, and how he started Smosh along with Ian.
Since his breakup with Cullen, Padilla did not continue video blogging, thus mostly disconnecting his personal life from his fan-base. However, he is now active on his personal channel alongside his girlfriend, Miel.
Anthony is currently dating Miel Bredouw, a former popular Viner under the name mielmonster, though not publicly announced by either of them. Miel owns a vegan bakery in LA and is also a singer. Anthony and Miel have traveled to Iceland, Boston, Vermont, Mexico, and various other places together.
Padilla has a self-named channel, on which he posts video blogs. The channel has had over 2 million subscribers since June 2017, the month he left Smosh.
Departure from Smosh
On June 14, 2017, it was announced that Anthony had left Smosh and had uploaded a video on his channel explaining the decision taken for it, he says that due to Smosh being owned by a company he could no longer express what he had in mind and was forced to do whatever was given to him. He had missed the time when he made Smosh to chat with his friends (then meeting Ian later) and wanted it to be independent.
- As stated in the two Smosh Pit videos "SEXIST BURGER", "ADDICTED TO BREAD", Anthony is of 25% (1/4) Filipino descent, since his paternal grandmother is from the Phillipines.
- Based on his DNA Test on his Twitter page, Anthony is 74% European, 24.2% East Asian & Native American, 1% Sub-Saharan African, 0.4% Middle Eastern & North African, and 1.2% is unassigned. 
- Anthony's parents were divorced when he was 2 and he was often jealous of kids who got to live with both their parents. He later had a stepdad who bailed out on them when he was 13.
- Anthony used to sleepwalk.
- When Anthony was 15, he was diagnosed with HSP. This caused Anthony to lose 30 pounds in just 2 weeks. Due to the disease, he was inable to go to school for a while. Anthony still has HSP, but it remains dormant.
- His first car was bought off of money generated by ads on Smosh.com.
- Anthony dropped out of College to work on YouTube full time.