The channel is generally run by Jason Steele who animates, song-writes and voice-acts most of their animated content. Most puppets and props used are created by "Scuffy". They both do some live acting, but not as much as the others. Chris Alex, Jennifer Alex, Nick Alex, and Robert Benfer often star in live action videos. When the channel first started it was common for Steele's and Alex's friends and family to act in their videos.
FilmCow usually creates different content every fortnight and as such don't have many series. Most of their animated series have stopped at three to four episodes ("Mystery of Westington Manner", "George and Angela", "Charlie the Unicorn"). Currently the longest animated series is "Llamas with Hats" which has ended with twelve episodes.
Live series are even less common than animated series being that right now there is only "Detective Heart of America", "Dark Midnight" and "Hit it With a Car". "Hit it With a Car" is their longest running live series (and series in general) with nine episodes.